We changed things up and were running some totally different things than they've seen. They saw some different things and it looked like they were a little confused, and our defensive line just put a lot of pressure on them.

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Our experimental results agreed with the computational findings, indicating that these ratchetting points mediate the removal of intron subfragments in one direction as the gene is transcribed initially from DNA into RNA.

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The striking evolutionary conservation of ratchetting points suggests that recursive splicing provides specific advantages for large introns, ... One possibility is that recursive splicing prevents the generation of long RNA transcripts that could form structures that interfere with correct processing into mRNA. Another is that recursive splicing might help stimulate trans.

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That's just our hogs stepping up the occasion.

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While some scientists have suspected that large introns might not be removed in one piece through direct splicing, no one had identified how this could happen. Now we have identified a way.

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We found that many large introns are removed by multiple recursive splicing steps, ... These steps involve the sequential excision of smaller subfragments. Our work also indicates that most recursive splicing events leave no clues in the final mRNA. This is why they have not been detected before now.

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